Caribou Biosciences Presents New Preclinical Data on CB-012, an Allogeneic Anti-CLL-1 CAR-T Cell Therapy, at the 2023 AACR Annual Meeting

Caribou

BERKELEY, Calif., April 17, 2023 - Caribou Biosciences, Inc. (Nasdaq: CRBU), a leading clinical-stage CRISPR genome-editing biopharmaceutical company, today presents a poster of preclinical data demonstrating the promise of CB-012, a next-generation CRISPR-edited allogeneic anti-CLL-1 CAR-T cell therapy, as a therapeutic candidate for adult patients with relapsed or refractory acute myeloid leukemia (r/r AML). The presentation takes place at the 2023 American Association for Cancer Research (AACR) Annual Meeting today from 1:30 pm to 5:00 pm EDT at the Orange County Convention Center, Orlando, Florida.  

Caribou’s patented next-generation CRISPR Cas12a chRDNA genome-editing technology platform, which maintains high genomic integrity and significantly improves the specificity of genome edits, was used to engineer the 5 genome edits implemented in the manufacture of CB-012. CB-012 is the first allogeneic CAR-T cell therapy, to Caribou’s knowledge, with both checkpoint disruption, through a PD-1 knockout (KO), and immune cloaking, through a B2M KO and B2M–HLA-E fusion transgene insertion. These armoring strategies were designed to promote the durability of antitumor activity. Preclinical data presented at the AACR meeting show:

  • CB-012 targets, becomes activated, proliferates, and demonstrates antitumor activity against a broad panel of AML cancer cell lines
  • Immune cloaking protects CB-012 from NK cell-mediated cytotoxicity
  • Mice harbouring AML xenograft models treated with CB-012 having a PD-1 KO showed extended survival relative to mice injected with control CAR-T cells that express PD-1 and that only contain 4 out of the 5 edits
  • CB-012 demonstrated significant antitumor efficacy and prolonged survival in AML xenograft models

Title: CB-012, an allogeneic anti-CLL-1 CAR-T cell therapy engineered with next-generation CRISPR technology to resist both the immunosuppressive tumor microenvironment and immune cell-mediated rejection, for patients with relapsed or refractory acute myeloid leukemia
Presenter: Tristan Fowler, PhD, associate director of preclinical pharmacology, Caribou Biosciences
Session Date: Monday, April 17, 2023
Presentation Time: 1:30 - 5:00 pm EDT
Abstract number: 3201

https://www.abstractsonline.com/pp8/#!/10828/presentation/3067

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